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BD FACSCaliburtm Item #1503
BD FACSCalibur: Rebuilt 4-color flow cytometer with G4 computer, Cellquest software, flat screen display and color printer. Sorting, Auto Loader and computer options are also available. This analyzer is capable of providing immunophenotyping, absolute counting, residual white blood cell enumeration, stem cell analysis, DNA analysis, and isolation by the sorting option. Provides cellular and molecular analysis in such fields as genetics, immunology, microbiology, pathology, toxicology, pharmaceuticals, urology, nephrology, epidemiology, bioengineering, proteomics and plant pathology.
 
Workstation: Macintosh G4 with OS9, CellQuest 3.5 software, new 22" flat screen display and color ink-jet printer. CellQuesttm software provides high-performance acquisition and analysis tools for plotting, gating, statistical analysis, and reporting. Can acquire up to 6 parameters and analyze 8 parameters (including time).
Lasers: 15mW 488 nm air-cooled argon, 635 nm red diode laser spatially separated for high sensitivity, minimal compensation, and maximum flexibility in fluorochrome selection.
Electronic doublet discrimination: Allows for the removal of artifacts from analysis by width and area measurements.
Fluorescence Sensitivity: <750 MESF
Coefficient of variation (CV): <3% full peak for propidium iodide–stained chicken erythrocyte nuclei.
Scatter resolution: Enables the separation of fixed platelets from noise and optimized for resolving lymphocytes, monocytes and granulocytes.
Compensation: Between FL1,FL2; FL2,FL3; FL3,FL4 controlled by hardware.
Minimum cell size: 0.2 microns.
Maximum analysis rate: 20,000 cells/sec.
Sample flow rates:12, 35 and 60 μL/min.
Power: 120 VAC +/- 10%, 50/60 Hz +/-2 Hz. Current: 15 amps maximum
Sensor module dimensions/weight: 36" w x 24" d x 26.5" h (49" high with cover open)/ 240lbs.
 

The following rebuilt options can be quoted separately:

Sorter: A unique catcher tube mechanism that moves in and out of the sample core stream 300 times per second to capture designated cells after they pass through the laser beam. The cells are then directed to collection tubes for further processing with >95% purity and >59% recovery. Sort modes of single cell, exclusion and recovery. This sorter has been confirmed to meet recovery and purity specifications.

Auto Loader: Tube lifter design with multiple sensors that verify rack ID and tube position. Holds 40 12x75 mm tubes per rack. Sample information for 16 racks can be defined through worklist manager software. Adjustable high-energy and low-energy mixing.

HTS Sampler: Acquires samples from 96- and 384-well plates (standard depth).

 
Thoroughly clean, remove corrosion and re-paint all interior and exterior panels as needed . Replace filters, fluidics tubing, connectors and valves as required. Clean, inspect, or replace waste and sheath tanks. Replace BAL seal. Remanufacture the 488nm laser and power supply. Replace any defective optical filters for FL1-FL4. Replace any defective photomultiplier and photodiode detectors. Replace the red diode laser if output less than 10mw. Clean flow cell or replace if needed. Confirm proper operation of droplet containment module (DCM). Set sample pressure to specifications. Adjust sample flow rates for correct settings. Clean (or replace) and align optics for optimum CVs using Redi-Check and Calibrite beads. Print all results to be included with shipment and/or emailed to you for approval. Confirm that all workstation and instrument functions meet original factory specifications.
 
Installation by a qualified service representative quoted separately. The price will include all travel expenses and labor required to confirm proper operation at the customer's facility utilizing standard calibration procedures. Any additional time required to correct malfunctions shall be included in the quoted installation fee.
 
Instrument and/or applications training by a qualified representative are available for an additional fee. The quoted price includes travel and perdiem expenses when training is performed during an installation visit. Otherwise additional reimbursement for travel and perdiem expenses may be required.
 
Custom warranty or service contracts can be quoted separately. All components are covered unless failure is due to operator negligence. In addition, we offer unlimited telephone support for all of the products sold through Spectron Corporation regardless of warranty.
 
Shipping costs will be quoted separately. If the purchaser becomes the shipping agent for one of the products located in the State of Washington they could be subject to paying Washington State sales tax unless exempt. No such obligation exists if Spectron Corporation is the designated shipper to destinations outside the State of Washington. This equipment shall be packed in a sturdy double-walled box surrounded inside by 2" foam sheets on all sides. All components shall be individually protected within the same container. The box will be taped, strapped and attached to a pallet with additional strapping. Shipping will be arranged through a qualified airfreight carrier with full insurance.
 
Most established institutions will be approved for net-30 terms or other payment plans. Leasing or rental programs may also be available for qualified buyers with various options for an eventual purchase. Washington State customers or others arranging shipment or picking up products themselves may be subject to Washington State sales tax unless exempt. Spectron Corporation will inform prospective buyers of any such circumstance that could result in a tax liability. Otherwise, buyers would only be subject to applicable use taxes that they might be required to pay directly to their respective state.
 
Spectron Corporation would appreciate the opportunity to bid on a service contract covering our products as well as other flow cytometers located at your facility. Please inquire into any modifications to the listed products that would better suit your requirements. We strive for complete satisfaction.
 

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