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BD LSRtm Item #1801
BD LSR serial number H0148: This 6-color, 8-parameter, 3- laser flow cytometer is capable of immunophenotyping, absolute counting, residual white blood cell enumeration, stem cell analysis and DNA analysis. Provides cellular and molecular analysis in such fields as genetics, immunology, microbiology, pathology, toxicology, pharmaceuticals, urology, nephrology, epidemiology, bioengineering, proteomics and plant pathology.
 
Workstation: Macintosh G4 with OS9, CellQuesttm3.5 software, new 22" flat screen display and color ink-jet printer. CellQuesttmsoftware provides high-performance acquisition and analysis tools for plotting, gating, statistical analysis, and reporting. Histogram analysis is provided by Modfittm LT (Verity Software House).
Lasers: Coherent 20 mW 488 nm air cooled argon, Kimmon 8 mW 325 nm HeCd and 17 mW 633 nm HeNe. Substitute lasers can be quoted separately.
Electronic doublet discrimination: Allows for the removal of artifacts from analysis by width and area measurements.
Fluorescence Sensitivity: 200 fluoroscein MESF using Spherotech Rainbow RCP-30-5A beads.
Coefficient of variation (CV): <3% full peak for propidium iodide or Hoechst 33342–stained chicken erythrocyte nuclei.
Fluorescence Compensation: Provided by FloJotm analysis software (Tree Star, Inc.)
Minimum cell size: 0.2 microns.
Maximum Analysis Rate: 20,000 cells/sec.
Sample flow rates:12, 35 and 60 μL/min.
Power: 120 VAC +/- 10%, 50/60 Hz +/-2 Hz. Current: 15 amps maximum
Sensor module dimensions/weight: 76" (193 cm) w x 34" (87 cm) d x 50" (127 cm) h / 525lbs (239 kg).
Manufacture date: 2001
 

Other lasers can be substituted for special applications such as a 561 nm diode laser for mCherry. Please request a quotation.

 
Performed instrument decontamination and flow cell cleaning procedures. Confirmed proper operation of droplet containment module (DCM). Replaced filters, fluidics tubing and connectors as required. Cleaned and inspected waste and sheath tanks. Replaced BAL seal. Set sample pressure to specifications. Conformed all lasers performing at required power and stability. Adjusted sample flow rates for correct settings. Cleaned and aligned optics for optimum CVs using Redi-Check and Calibrite beads. Printed all results to be included with shipment and/or faxed to purchaser for approval. Confirmed that all workstation and instrument functions meet original factory specifications.
 
Installation by a qualified service representative quoted separately. The price will include all travel expenses and labor required to confirm proper operation at the customer's facility utilizing standard calibration procedures. Any additional time required to correct malfunctions shall be included in the quoted installation fee.
 
Instrument and/or applications training by a qualified representative are available for an additional fee. The quoted price includes travel and perdiem expenses when training is performed during an installation visit. Otherwise additional reimbursement for travel and perdiem expenses may be required.
 
Custom warranty or service contracts can be quoted separately. All components are covered unless failure is due to operator negligence. In addition, we offer unlimited telephone support for all of the products sold through Spectron Corporation regardless of warranty.
 
Shipping costs will be quoted separately. If the purchaser becomes the shipping agent for one of the products located in the State of Washington they could be subject to paying Washington State sales tax unless exempt. No such obligation exists if Spectron Corporation is the designated shipper to destinations outside the State of Washington. This equipment shall be packed in a sturdy double-walled box surrounded inside by 2" foam sheets on all sides. All components shall be individually protected within the same container. The box will be taped, strapped and attached to a pallet with additional strapping. Shipping will be arranged through a qualified airfreight carrier with full insurance.
 
Most established institutions will be approved for net-30 terms or other payment plans. Leasing or rental programs may also be available for qualified buyers with various options for an eventual purchase. Washington State customers or others arranging shipment or picking up products themselves may be subject to Washington State sales tax unless exempt. Spectron Corporation will inform prospective buyers of any such circumstance that could result in a tax liability. Otherwise, buyers would only be subject to applicable use taxes that they might be required to pay directly to their respective state.
 
Spectron Corporation would appreciate the opportunity to bid on a service contract covering our products as well as other flow cytometers located at your facility. Please inquire into any modifications to the listed products that would better suit your requirements. We strive for complete satisfaction.
 

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